Interscience for microbiology
 

A complete range from sampling to counting

Labo 3D - Santé animale EN

1 Collect

1 Collect

The analysis begins with on-site and/or on production line collection. The flexibility of our range of RollBag bags can be adapted to any type of test (biopsies, biological samples, etc.).

2Sample

2Sample

Sampling for microbiological analysis preparation. The sample is placed in its unique bag with filter (BagPage 100 mL) for blender avoiding cross contamination. The bag stands upright with a BagOpen.

3Dilute

3 Dilute

Weigh the sample with the DiluFlow Elite 1 kg dilutor. The DiluFlow dilutor automatically dispenses the diluent according to the chosen dilution factor with ± 2% accuracy in accordance with the standards.

4 Homogenize

4Homogenize

The sample is homogenised in a MiniMix without any contact with the blender, thus avoiding cross-contamination. Bacteria are quickly extracted from the sample without being destroyed and the filter in the bag removes particles in the filtrate.

5Organize

5Organize

The homogenised samples are then stored in a BagRack.

6Distribute

6Distribute

With FlexiPump, the serial distribution of culture media, agars, diluents... is accurate, fast and sterile.

7Plate

7Plate

Place the filtered sample in a beaker.
The easySpiral plater automatically plates the sample on a Petri dish, allowing 4 logs of dilution to be carried out on the same dish. The easySpiral software collects the plating data.

8Count

8Count

Once the incubation is complete, the automatic counting is performed with the Scan range automatic colony counters. Click on “Count” then “Validate”. The images and results are saved automatically on your computer to ensure traceability.

Types of analyses

  • Animal health and R&D
    • Biopsies
    • Biological sampling
    • Study of a bovine tuberculosis outbreak
    • Antibiograms and antibiotic dosages in farming
    • Enumeration of normal and infectious flora specimens
  • Veterinary industry
    • Production quality control
    • Challenge test
    • Environment

Example of protocol in the veterinary sector

Collect the sample with RollBag

Collect the sample with RollBag

Dilute the sample at 1/10th with DiluFlow

Weigh 10 g biopsy sample and dilute with DiluFlow Elite 1 kg

Homogenize with MiniMix lab blender

Homogenize and filter the sample in a MiniMix

Plate with easySpiral

Plate the filtrate with easySpiral Dilute

Incubate Petri dishes with ScanStation

Place the Petri dishes in ScanStation for incubation

Obtain a compliance result with ScanStation

Enumerate the colonies to obtain a result with ScanStation or Scan for a preventive or curative diagnosis

Collect the sample with RollBag

Collect the sample with RollBag

Dilute the sample at 1/10th with DiluFlow

Weigh 20-50 g of faecal sample and dilute with DiluFlow

Homogenize with MiniMix lab blender

Homogenize and filter the sample in a BagMixer

Plate with easySpiral

Plate the filtrate using the constant mode with easySpiral and place the antibiotic discs before incubation

Interpret Petri dishes (SIR) with Scan

Read and interpret (SIR) multi-resistance according to the veto CASFM with the Scan range

Products for veterinary analysis

Sterile sampling bags

Blender bags with filter

Volumes: 100-400 mL

Blender bags without filter

Volumes: 100-400 mL

Lab accessories

Gravimetric dilutors

MiniMix 100 P CC

MiniMix 100 W CC

BagMixer 400 P

BagMixer 400 W

BagMixer 400 CC

BagMixer 400 S

BagMixer 400 SW

Dispensing pumps

Automatic platers

Colony counters

Inhibition zone readers

Real-time incubator and colony counter

In accordance with the directives

CASFM vétérinaire

Antibiotics are essential as veterinary drugs in the treatment and control of infectious animal diseases of bacterial etiology. The development of recommendations for the performance of antibiotic susceptibility testing in veterinary diagnostics is intended to provide information suitable for veterinary medicine.

By creating a veterinary sub-working group, the Antibiogram Committee wished to contribute to the implementation of a reasoned approach to the use of this therapeutic class by veterinarians. The comparison of the experience of the various members of the committee has made it possible to draw up this list of critical thresholds. These are defined by bacterial species and distinguish between phenotypes sensitive and resistant to antibiotics available for veterinary practice.

Application notes

  • Dispensing repeatability of 9 mL from a 2-liter bottle with FlexiPump

    Aim: Evaluate the repeatability of a volume dispensed consecutively, after a FlexiPump calibration, from a FlexiPump calibration from a 2L diluent bottle (tube weight at the bottom and bottle filled for calibration).

    Conclusion: From the results obtained in this test, we can conclude that dispensing successive doses with the FlexiPump, from a 2-liter bottle of diluent, shows excellent repeatability. There is no drift in dispensing volume as the bottle empties.

  • Dispensing repeatability of 9 mL diluent bag with FlexiPump

    Aim: Evaluate the repeatability of a consecutively dispensed volume, after instrument calibration, from a 2L diluent bag.

    Conclusion: From the results obtained in this test, we can conclude that dispensing successive doses with the FlexiPump, from a diluent bag, shows excellent repeatability. There is no drift in dispensing volume as the bag empties.

  • 50 ML dispensing test with FlexiPump Pro

    Aim: Check the dispensing accuracy of a 50 mL volume with a FlexiPump Pro peristaltic pump, with a dispensing time of less than 4/5 seconds.

    Conclusion: The FlexiPump Pro is accurate for a 50 mL dispense, under the conditions described above. Note that under these conditions, each dispense took 2.7s.

  • Scan 1200: performance evaluation

    Aim: The aim of this study was to evaluate the performance of the Scan 1200 by comparing manual and automatic counting. For optimal comparison, Petri dishes were plated and incubated in our R&D laboratory, using standard methods to reproduce normal laboratory conditions. The same technician then counted the colonies with a Scan 1200 and manually to obtain results for evaluating the accuracy of the Scan. This document also contains a study of the analysis time per dish and an estimate of the time spent by the laboratories.

    Conclusion: Tests show in a variety of ways (regression line, correlation coefficient, average Log value difference, and ISO 7218:2007) that Scan 1200:
    — Enables faster counting (up to 80% time saving).
    — Counts as well as another user (strong relationship between the 2 methods, with an average difference of 2.35% per dish).

    The Scan 1200 is an excellent tool for laboratories needing to count large numbers of dishes accurately and without wasting time. All results can be saved in specific files (called sessions) which contain all dish photos and counts, guaranteeing analysis quality and perfect traceability.

  • Counting waterborne pathogens with ScanStation, a real-time incubation and counting station. Use of filtration membranes

    Aim: The objective of this study was to evaluate the performance of the ScanStation to count in real time colonies on membrane filtration. Enumeration was performed on waterborne pathogens that have been linked to healthcare-associated infections (HAIs). Bacterial suspensions were passed through filtration membranes that were deposited on Petri dish. Colonies were manually counted and results were compared with automatic counts performed by the ScanStation.

    Conclusion: ScanStation performs well in counting colonies on filtration membranes in real time. For the seven strains tested, the automatic and manual counts are similar when bacterial suspensions are filtered on white polycarbonate membranes (without grids). For best automatic counts in this case, the recommended light configuration is white background (light from below). This study shows that bacterial colonies can be efficiently counted with ScanStation.

  • Robustness tests on ScanStation

    Aim: The aim of this study is to evaluate the performance of ScanStation (ISS) by comparing manual and automatic counting of plated samples for robustness counting assessment.

    Conclusion: ScanStation’s robustness tests showed reproducible data under both intra- and inter-machine conditions.

They trust us

Logo Oniris

ONIRIS
National School of Veterinary Medicine and Food Processing, France

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Dilute up to 10-8 without any possibility of error
Logo LVD 06

LVD 06
Departmental Veterinary Laboratory of the Maritime Alps, France

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The Spiral plater in service for more than 33 years!
Logo Vetoquinol

Vetoquinol
Veterinary pharmaceutical laboratory, international lab

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This saves precious time, and less handling
Logo LVD 82

LVD 82
Tarn et Garonne Departmental Veterinary Laboratory

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Adjusting BagMixer’s paddles allows us to adjust the blending force according to our sample

The different industries of our applications

Our products are also used for microbiological analyses in food, pharmaceutical, cosmetic, environmental industries and public institutes research.

Food

Pharmaceutical

Cosmetic

Environment

Public research

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